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Streptavidin, Fluorescein
Description
Streptavidin, fluorescein (FITC) can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with?our highly purified fluorescein?labeled streptavidin.
Features:
- Recommended for routine immunofluorescence applications
- Extremely high affinity for biotin
- Has an optimal fluorochrome to protein ratio
- Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
- Excitation maximum at 495 nm
- Emission maximum at about 515 nm
- Color:? Green
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | For diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30 μg/ml. |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Excitation | 495-500 nm |
| Maximum Emission | 514-521 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 1.0 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Anti-Streptavidin Antibody, Biotinylated Catalog #: BA-0500-.5
Streptavidin/Biotin Blocking Kit Catalog #: SP-2002
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Citations
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