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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命，該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。
Streptavidin, Fluorescein
Description
Streptavidin, fluorescein (FITC) can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with?our highly purified fluorescein?labeled streptavidin.
Features:
- Recommended for routine immunofluorescence applications
- Extremely high affinity for biotin
- Has an optimal fluorochrome to protein ratio
- Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
- Excitation maximum at 495 nm
- Emission maximum at about 515 nm
- Color:? Green
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | For diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30 μg/ml. |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Excitation | 495-500 nm |
| Maximum Emission | 514-521 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 1.0 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Anti-Streptavidin Antibody, Biotinylated Catalog #: BA-0500-.5
Streptavidin/Biotin Blocking Kit Catalog #: SP-2002
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Citations
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