Vector Lab代理Streptavidin, DyLight? 488

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Streptavidin, DyLight? 488

SA-5488-1

SKU Unit Size Price Qty
SA-5488-1 1 mg

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Streptavidin, DyLight? 488

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.?

Features:

  • Advantages of DyLight? dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum:?493 nm
  • Emission maximum: 518 nm
  • Color:? Green

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 μg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 493 nm
Maximum Emission 518 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 488
Color of Fluorescence Green
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

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Citations

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Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin.?

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight? dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight? conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight? conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

*Additional information about the images can be found here.

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Streptavidin, DyLight? 488

Streptavidin, DyLight? 488

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