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Streptavidin, DyLight? 488
Description
DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.?
Features:
- Advantages of DyLight? dyes include greater photostability, pH independence, and brighter fluorescence
- Excitation maximum:?493 nm
- Emission maximum: 518 nm
- Color:? Green
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | Recommended concentration range for use: 5-30 μg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Excitation | 493 nm |
| Maximum Emission | 518 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 1.0 mg/ml active conjugate |
| Conjugate | DyLight 488 |
| Color of Fluorescence | Green |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Anti-Streptavidin Antibody, Biotinylated Catalog #: BA-0500-.5
Streptavidin/Biotin Blocking Kit Catalog #: SP-2002
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Citations
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Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin.?
Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.
DyLight? dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight? conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight? conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.
*Additional information about the images can be found here.
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