Megazyme K-YBGL Beta葡聚糖[酵母和蘑菇]檢測試劑盒

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Megazyme K-YBGL Beta葡聚糖[酵母和蘑菇]檢測試劑盒

Megazyme代理，部分現貨，期貨產品2-3周

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產品名稱：Beta葡聚糖[酵母和蘑菇]檢測試劑盒

英文名稱：Mushroom and yeast beta-glucan assay Kit

貨號： K-YBGL

型號規格：100次

優點：成本低；所有試劑配制后的穩定性〉2年；只有酶試劑盒可用；；方法簡單；包含標準品

檢測原理：

檢測方法：比色法

檢測波長：510nm；

總檢測時間：100min；

檢測限：樣品重量的1-100%；

適用樣品：酵母制備、蘑菇制備及其他原料；

方法認證：新方法

商品說明：

For the measurement of 1,3:1,6-?-glucan and α-glucan in yeast preparations. Content: 100 assays per kit

Colourimetric method for the determination of Yeast and?
Mushroom β-Glucan in yeast, mushroom, foodstuffs and?
other materials

Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O →?
soluble glucan

(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)

(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose

(glucose oxidase)
(4) D-Glucose + H2O + O2?→ D-gluconate + H2O2

(peroxidase)
(5) 2H2O2?+ p-hydroxybenzoic acid + 4-aminoantipyrine →?
?quinoneimine + 4H2O

(amyloglucosidase)
(6) α-Glucan + H2O ? ?→ ? ?D-glucose

Kit size: ?????????????? ????????????100 assays
Method: ???????????? ??????????????Spectrophotometric at 510 nm
Total assay time:?????????? ???~ 100 min
Detection limit:??????????????? ?1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition: ?? ????Novel method

Advantages

  • Very cost effective
  • All reagents stable for > 12 months after preparation
  • Only enzymatic kit available
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included