Megazyme K-SUFRG 蔗糖/D-果糖/D-葡萄糖檢測(cè)試劑盒
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產(chǎn)品名稱:蔗糖/D-果糖/D-葡萄糖檢測(cè)試劑盒
英文名稱:Sucrose,D-fructose and D-glucose sucrose assay kit
貨號(hào):K-SUFRG
型號(hào)規(guī)格:150 (50 of each)
優(yōu)點(diǎn):價(jià)格低廉(每次檢測(cè)成本);
所有試劑配制后的穩(wěn)定性〉12個(gè)月;
反應(yīng)快;葡萄糖氧化酶/過(guò)氧化酶/己糖激酶/6-磷酸葡萄糖脫氫酶;
包含標(biāo)準(zhǔn)品
檢測(cè)原理:

檢測(cè)方法:紫外分光光度法
檢測(cè)波長(zhǎng):340nm;
反應(yīng)時(shí)間:30min;
檢測(cè)限:1.38mg/L;
適用樣品:啤酒、果汁、軟飲料、牛奶、果醬、蜂蜜、面包、焙烤產(chǎn)品、乳制品、糖果、甜點(diǎn)、糖食、冰淇淋、水果和蔬菜(如:馬鈴薯)、肉制品(如香腸)、調(diào)味品、飼料、煙、化妝品、紙及其他原料(如生物培養(yǎng)、樣品等);
方法認(rèn)證:EN。NEN。NF。DIN,GOST,IFU ,AIJN,MEI,IOCCC以及德國(guó)、奧地利認(rèn)證
The Sucrose/Fructose/D-Glucose test kit is suitable for the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of Sucrose, D-Fructose and
D-Glucose in foodstuffs, beverages and other materials
Principle:
(β-fructosidase)
(1) Sucrose + H2O → D-glucose + D-fructose
(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphoglucose isomerase)
(5) F-6-P → G-6-P
Kit size: 100 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 1.38 mg/L
Application examples:
Beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread,
bakery products, dairy products, candies, desserts, confectionery, sweets,
ice-cream, fruit and vegetables (e.g. potato), meat products (e.g. sausage),
condiments (e.g. ketchup and mustard), feed, tobacco, cosmetics,
pharmaceuticals, paper and other materials
Method recognition:
Methods based on this principle have been accepted by NF, EN, NEN,
DIN, GOST, IFU, AIJN, MEBAK and IOCCC
Advantages
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Stabilised D-glucose / D-fructose standard solution included
- Extended cofactors stability