Vector Lab代理Methyl Green Counterstain

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Methyl Green Counterstain

H-3402-500

SKU Unit Size Price Qty
H-3402-500 500 ml

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Methyl Green Counterstain

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.

Features:

  • Simple, two-step procedure
  • Suitable for use with a wide range of enzyme substrates
  • Suitable for use with non-aqueous mountants

Specifications

More Information
Unit Size 500 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

Documents

  • User Guide
  • Counterstain/Substrate Compatibility Chart
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

I am using Methyl Green (H-3402) as a counterstain for my IHC, but the stain is a little weak. What suggestions do you have to increase the staining intensity?

Methyl Green counterstain does take a little bit of optimization in some applications. We would suggest heating a volume of the counterstain (~300 ml) to 60 °C and add to a Coplin jar or similar glass staining rack, and submerse the slides. This approach facilitates a better uptake of stain into the section compared with placing the slide on a heated surface and placing drops of stain onto the section. Following the incubation time (~3-5 min), remove slides and wash in tap water. Omit the acetone, acetic acid rinse step described in the instructions and move the slides directly into 95% ethanol for the dehydration, clearing and mounting process. This methodology will retain more stain in the nuclei and hence produce greater intensity.

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Citations

Methyl Green Counterstain Powered by Bioz See more details on Bioz

Technical Information

Counterstain/Substrate Compatibility Table

This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.

Methyl Green Counterstain

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