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Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Biotinylated
Description
GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin. Biotinylated GSL I has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs |
| Recommended Usage | For most applications we recommend a freshly prepared working solution of 5-20 μg/ml in the above buffer. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Inhibiting and/or Eluting Sugar | Inhibiting/Eluting Sugar: Mixture of 200 mM galactose (S-9003)/200 mM N-acetylgalactosamine (S-9001). |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2. |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Galactose, N-Acetylgalactosamine |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
N-acetylgalactosamine Catalog #: S-9001
Galactose Catalog #: S-9003
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Unconjugated Catalog #: L-1100-5
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Rhodamine Catalog #: RL-1102-2
Citations
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This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine