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Streptavidin, Alkaline Phosphatase

SA-5100-1

SKU	Unit Size	Price	Qty
SA-5100-1	1 ml

Description

Specifications

Documents

Description

Alkaline Phosphatase?Streptavidin (AP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of alkaline phosphatase. Using biotinylated primary or secondary intermediates and alkaline phosphatase-conjugated streptavidin, it is suitable for use in solid-phase?assays, tissue/cell staining systems, and blotting applications.

Features:

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
Vector Laboratories’ enzyme-conjugated avidin and streptavidin?are produced with the highest specific activity enzymes in?optimal ratios
Specific covalent linkages are chosen to provide?stable, highly active conjugates

Specifications

More Information
Unit Size	1 ml
Applications	Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage	The recommended dilution for use is 1:200 to 1:1000.(1-5 μg/ml). Storage of dilute solutions of the enzyme conjugate is not recommended.To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1mg/ml immunohistochemical grade bovine serum albumin (Catalog No. SP-5050).DO NOT USE FRACTION V GRADE BSA.
Solution	10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl₂, 1% sucrose, 2 mg/ml bovine serum albumin.
Recommended Storage	2-8 °C
Detection Enzyme(s)	Alkaline Phosphatase
Conjugate	Alkaline Phosphatase
Format	Concentrate