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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命，該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。
Vector? VIP Substrate Kit, Peroxidase (HRP)
Description
The?Vector?VIP?HRP substrate?produces?an?intense, violet (purple) reaction product, and can be used as an alternative to DAB or as a second color for multiple antigen labeling.
Features:
- Greater sensitivity than conventional substrates
- Consistent and reliable
- Ideal for IHC/ICC/ISH and blots
- Permanent mounting
- Stock solutions supplied in convenient dropper bottles promoting ease of handling
- No wait times for mixing and dissolving powders or tablets
- Sufficient reagents to produce 300 ml of working solution
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Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Peroxidase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Purple |
| Microscopy | Brightfield, Darkfield, Electron, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Multiple Labeling |
| Mounting | Non-Aqueous (Permanent) |
Kit Components
Kit Contents:
- 7 ml Vector VIP Reagent 1
- 5 ml Vector VIP Reagent 2
- 5 ml Vector VIP Reagent 3
- 7.2 ml Vector VIP Reagent 4
Documents
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
BLOXALL? Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Methyl Green Counterstain Catalog #: H-3402-500
Hematoxylin Counterstain Catalog #: H-3401-500
Hematoxylin QS Counterstain Catalog #: H-3404-100
VectaMount? Permanent Mounting Medium Catalog #: H-5000-60
Citations
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Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.
Vector VIP Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector VIP substrate is equivalent to Vector DAB Substrate. This product can also be used on blots. The kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4° C, this kit is stable for one year.
Product review on Biocompare.com.
1. Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122
