Vector Lab代理Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated

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Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated

B-1215-2

SKU Unit Size Price Qty
B-1215-2 2 mg

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Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Griffonia (Bandeiraea) Simplicifolia Lectin II is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linkedN-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of?N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked?N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.?

Biotinylated GSL II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

More Information
Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage Reconstitute biotinylated lectin by addition 1 ml of water.The resulting solution will have the following composition:10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 10 mM N-Acetylglucosamine.For most applications, we recommend a freshly prepared working solution of 5-20 μg/ml in the above buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Conjugate Biotinylated
Sugar Specificity N-Acetylglucosamine

Documents

  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Related Products

Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated

Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Unconjugated Catalog #: L-1210-5

Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated

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Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated

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Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Fluorescein Catalog #: FL-1211-2

Citations

Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine

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