Vector Lab代理Ulex Europaeus Agglutinin I (UEA I), Biotinylated

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Ulex Europaeus Agglutinin I (UEA I), Biotinylated

B-1065-2

SKU Unit Size Price Qty
B-1065-2 2 mg

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Ulex Europaeus Agglutinin I (UEA I), Biotinylated

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Ulex europaeus?agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

Biotinylated?Ulex europaeus?agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

More Information
Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications we recommend a freshly prepared working solution of 5-20 μg/ml in the above buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide.
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Fucose, Arabinose

Documents

  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

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Citations

Ulex Europaeus Agglutinin I (UEA I), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 50 mM - 100 mM L-fucose

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