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Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, Fluorescein
Description
GSL I-B4?isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. ?This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.?
Fluorescein labeled GSL I-B4?has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorescein and is preserved with sodium azide.?
- Excitation maximum = 495 nm
- Emission maximum = 515 nm
- Color = green
Specifications
| Unit Size | 500 ug |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 μg/ml. |
| Recommended Storage | 2-8°C |
| Maximum Excitation | 495-500 nm |
| Inhibiting and/or Eluting Sugar | 500 mM galactose (S-9003) or 100 mM raffinose |
| Maximum Emission | 514-521 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 |
| Concentration | 1 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Sugar Specificity | Galactose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Galactose Catalog #: S-9003
Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, Unconjugated Catalog #: L-1104-1
Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight? 594 Catalog #: DL-1207-.5
Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, Biotinylated Catalog #: B-1205-.5
Citations
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Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting Sugar: 500 mM galactose or 100 mM raffinose