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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命，該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。
M.O.M.? (Mouse on Mouse ) Elite? Immunodetection Kit, Peroxidase
Description
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The M.O.M.?(Mouse on Mouse ) Elite Peroxidase Kit significantly reduces?endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue. It is based on VECTASTAIN? ABC reagent protocols.
Key advantages of the Vector M.O.M. Immunodetection Kits:
- Clear, crisp specific staining of antigens of interest
- Procedures are simple and easy to follow
- No tedious calculations or antibody prebinding steps required
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The Vector M.O.M. Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue sections.
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Blotting Applications |
| Technology | ABC Elite (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems |
| Detection Enzyme(s) | Peroxidase |
| Target Species | Mouse |
| Format | Concentrate |
| Blocking Action | Endogenous Mouse Ig |
Kit Components
Kit Contents:
- 6 ml of M.O.M. Protein Concentrate
- 1 ml M.O.M. Mouse Ig Blocking Reagent
- 0.1 ml M.O.M. Biotinylated Anti-Mouse IgG Reagent
- VECTASTAIN Elite ABC Reagents A(1 ml) and B (1 ml)
Documents
- User Guide
- M.O.M. (Mouse on Mouse) Troubleshooting Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
For my IHC application, I intend to apply a mouse IgG primary antibody on rat tissue sections. Should I use the Vector Mouse on Mouse detection kit?
Should the VECTASTAIN ABC kit only be stored at 4 degrees C? Can it be stored at -20 degrees C?
We do not recommend freezing the ABC kits. In particular, the ABC reagents exhibit a decrease in enzyme activity and lose some sensitivity when stored at – 20 °C for extended periods.
Related Products
Avidin/Biotin Blocking Kit Catalog #: SP-2001
M.O.M.? (Mouse on Mouse) Blocking Reagent Catalog #: MKB-2213-1
M.O.M.? (Mouse on Mouse) Biotinylated Anti-Mouse IgG Reagent Catalog #: MKB-2225-.1
VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
ImmPACT? DAB Substrate, Peroxidase (HRP) Catalog #: SK-4105
ImmPACT? VIP Substrate, Peroxidase (HRP) Catalog #: SK-4605
ImmPACT? SG Substrate, Peroxidase (HRP) Catalog #: SK-4705
ImmPACT? NovaRED? Substrate, Peroxidase (HRP) Catalog #: SK-4805
ImmPACT? AMEC Red Substrate, Peroxidase (HRP) Catalog #: SK-4285
ImmPACT? AEC Substrate, Peroxidase (HRP) Catalog #: SK-4205
Citations
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The Vector?M.O.M. Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a?Vector?M.O.M. Elite?Kit. The result is clear, crisp specific staining of the antigens of interest.
The?Vector?M.O.M. Elite?Kit contains a proprietary Mouse IgG Blocking Reagent and is based on the simple and easy to follow?VECTASTAIN??ABC reagent protocols; no tedious calculations or antibody pre-binding steps are required. Excellent staining results for a once difficult application have now become routine with the?Vector?M.O.M. System.
The Vector?M.O.M. Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts. ?The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, "Discovery Through Color: A Guide to Multiple Antigen Labeling" , available on our website.
Four Vector?M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.
The Vector?M.O.M. ImmPRESS Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS Peroxidase Polymer reagent.
Choose from a wide variety of peroxidase substrates available for chromogenic development.
Product review on Biocompare.com.