Vector Lab代理5′ EndTag Nucleic Acid Labeling Kit

發表于由

Vector Labs代理，歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命，該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

5′ EndTag Nucleic Acid Labeling Kit

MB-9001

SKU Unit Size Price Qty
MB-9001 1 kit

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

5' EndTag Nucleic Acid Labeling Kit

Description
Specifications
Kit Components
Documents
Related Products
Citations
Technical Information

Description

End labeling is a favored method for applications in which an internal label might interfere with hybridization or sequence specific protein binding.

Features of the 5′ EndTag DNA/RNA Labeling Kit:

  • Enables simple and uniform labeling of the 5′ end of a DNA, RNA or unmodified oligonucleotide molecule.
  • Preserves the unmodified 3′ end of the oligonucleotide (ideal for the labeling of PCR primers as a result).
  • Compatible with a choice of diverse labels – fluorophores, biotin, haptens, or affinity ligands*

Specifications

More Information
Unit Size 1 kit
Target for Labeling DNA, Oligonucleotides, RNA
Required Reactive Group 5 ‘-OH group of nucleic acid
Tag/Group Incorporated Thiol
Safety Title WARNING: NA – www.P65Warnings.ca.gov

Kit Components

What’s in the box?

Materials suitable to perform 10 end labeling reactions of up to 0.6 nmols of 5′ ends (e.g. about 5 μg of a 25 base oligo) per reaction. This includes:

  • T4 polynucleotide kinase
  • 10x reaction buffer
  • ATPγS
  • Precipitant
  • Alkaline Phosphatase

*Note: Thiol-reactive labeling reagent is not included in the kit and must be purchased separately. Labels suitable for this kit include:

  • Biotin (Long Arm) Maleimide
  • DNP Maleimide
  • Fluorescein (Long Arm) Maleimide
  • Fucose Maleimide
  • Texas Red? Maleimide

Note regarding shipping: This item is shipped on wet ice and therefore requires additional shipping charges.

Documents

  • User Guide
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

5' EndTag Nucleic Acid Labeling Kit

Biotin (Long Arm) Maleimide Catalog #: SP-1501-12

5' EndTag Nucleic Acid Labeling Kit

Fluorescein Maleimide Catalog #: SP-1502-12

5' EndTag Nucleic Acid Labeling Kit

Texas Red Maleimide Catalog #: SP-1505-3.6

5' EndTag Nucleic Acid Labeling Kit

3' EndTag DNA End Labeling System Catalog #: MB-9002

5' EndTag Nucleic Acid Labeling Kit

PHOTOPROBE? Biotin for Nucleic Acid Labeling Catalog #: SP-1000

5' EndTag Nucleic Acid Labeling Kit

PHOTOPROBE? (Long Arm) Biotin for Nucleic Acid Labeling Catalog #: SP-1020

5' EndTag Nucleic Acid Labeling Kit

QuantTag Biotin Quantitation Kit Catalog #: BDK-2000

Citations

5' EndTag Nucleic Acid Labeling Kit Powered by Bioz See more details on Bioz

Technical Information

End labeling is a favored method for applications in which the presence of an internal label might interfere with hybridization or sequence specific protein binding.

5' or 3' EndTag labeled nucleic acids can be used for applications such as DNA hybridization, PCR, in situ hybridization, the binding of capture probes to affinity matrices, or electrophoretic mobility shift assays (EMSA). Short oligonucleotides are labeled more efficiently with these systems than with other methods. In addition, end labeling of oligonucleotides is an economical alternative to having labels inserted during synthesis.

Both the 5' EndTag and 3' EndTag Nucleic Acid Labeling Systems enable the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the respective ends of a nucleic acid using thiol-specific chemistry. Labels containing thiol-reactive groups (maleimides, iodoacetamides, etc.) can easily be incorporated.

Labeling time is about 1 hour with very little hands-on time.

5' EndTag Labeling Kit

The 5' EndTag System labels 5' ends of DNA, RNA, or unmodified oligonucleotides. This kit is ideal for labeling PCR primers because a label is attached only to the 5' end, leaving the 3' end available for polymerization.

Labeling with the 5' EndTag Kit is achieved in two steps:

  1. T4 polynucleotide kinase transfers a thiophosphate from ATPγS to the 5' -OH group of the nucleic acid. (5' phosphorylated ends are converted to 5' -OH groups with the included alkaline phosphatase).
  2. Thiolated sample is coupled to a thiol-reactive label. The 5' EndTag Kit is designed to perform 10 labeling reactions of up to 0.6 nmols of 5' ends (e.g. about 5 μg of a 25 base oligo) per reaction. Thiol-reactive label is not included in the kit and should be selected separately.
5' EndTag Nucleic Acid Labeling Kit

5' EndTag Nucleic Acid Labeling Kit
Figure 1: EtBr-stained gel of a 1002 bp PCR product amplified using:
Ln 1) Unlabeled primers
 Ln 2) EndTag biotinylated forward primer and unlabeled reverse primer
 Ln 3) Unlabeled forward primer and EndTag biotinylated reverse primer
 Ln 4) EndTag biotinylated forward and reverse primers.

5' EndTag Nucleic Acid Labeling Kit
Figure 2: Transmission electron micrographs of molecules of influenza A viral ribonucleoprotein particles (vRNPs) labeled at the 5' end of the vRNA with biotin using 5' EndTag Kit, and further labeled with streptavidin gold. vRNPs from influenza A were purified according to Kemler et al., on a glycerol gradient with modifications as described in Wu et al. Courtesy of Drs. Winco WH Wu and Nelly Panté, University of British Columbia, Vancouver BC, Canada.

5' EndTag Nucleic Acid Labeling Kit
Figure 3: Nuclear import assay in digitonin-permeabilized HeLa cells of biotinylated vRNPs. vRNPs were labeled first at the 5' end of the vRNA with biotin using the 5' EndTag Kit and then with Vector Fluorescein Streptavidin. This allowed for direct fluorescence visualization of the vRNPs on a confocal fluorescence microscope. Nuclear import assays were carried out as described in Wu et al. The negative control consists of vRNPs added to the cells in the absence of energy and exogenous cytosol. In the presence of energy and cytosol, the fluorescein-labeled vRNPs successfully enter the nucleus, with a high degree of nucleolar staining. Courtesy of Drs. Winco WH Wu and Nelly Panté, University of British Columbia, Vancouver BC, Canada.

5' EndTag Nucleic Acid Labeling Kit
Figure 4: Fluorescence in situ hybridization of human chromosomes using 5' EndTag Fluorescein-labeled pUC1.77 detected directly and mounted in Vectashield Mounting Media with PI.

5' EndTag Nucleic Acid Labeling Kit
Figure 4: Coherent anti-Stokes Ramen scattering (CARS) and two-photon fluorescence (TPF) imaging of fluorescein-labeled HCV RNA inside Huh-7 cells. HCV RNA in-vitro transcripts were labeled with a fluorescein-maleimide label using the 5' EndTag Nucleic Acid Labeling System. Huh-7 were transfected with 3μg of 5'-fluorescein-labeled HCV RNA and imaged at 4 h post-transfection. CARS and TPF of Mock transfected Huh-7 cells. Figure courtesy of Jennifer Haley, Sylvie Belanger, Adrian Pegoraro, Albert Stolow, and John P. Pezacki, The Steacie Institute for Molecular Sciences, The National Research Council of Canada. Unpublished data.

5' EndTag Nucleic Acid Labeling Kit
Figure 5: Coherent anti-Stokes Ramen scattering (CARS) and two-photon fluorescence (TPF) imaging of fluorescein-labeled HCV RNA inside Huh-7 cells. HCV RNA in-vitro transcripts were labeled with a fluorescein-maleimide label using the 5' EndTag Nucleic Acid Labeling System. Huh-7 were transfected with 3μg of 5'-fluorescein-labeled HCV RNA and imaged at 4 h post-transfection. Here, CARS and TPF of Huh-7 cells transfected with fluorescein-labeled HCV RNA. Figure courtesy of Jennifer Haley, Sylvie Belanger, Adrian Pegoraro, Albert Stolow, and John P. Pezacki, The Steacie Institute for Molecular Sciences, The National Research Council of Canada. Unpublished data.

5' EndTag Nucleic Acid Labeling Kit
Figure 7: Fluorescence in situ hybridization of human chromosomes using 5' EndTag Texas Red-labeled pHuR 98 detected directly and mounted in Vectashield Mounting Media with DAPI.

 

Home All Products Conjugation Kits 5' EndTag Nucleic Acid Labeling Kit