Vector Lab代理Wisteria Floribunda Lectin (WFA, WFL), Biotinylated

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Wisteria Floribunda Lectin (WFA, WFL), Biotinylated

B-1355-2

SKU Unit Size Price Qty
B-1355-2 2 mg

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Wisteria Floribunda Lectin (WFA, WFL), Biotinylated

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

The binding specificity of?Wisteria floribunda?lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in?N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Biotinylated WFL has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

More Information
Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 μg/ml in the above buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity N-Acetylgalactosamine

Documents

  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Related Products

Wisteria Floribunda Lectin (WFA, WFL), Biotinylated

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound Catalog #: AL-1353-2

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Wisteria Floribunda Lectin (WFA, WFL), Fluorescein Catalog #: FL-1351-2

Wisteria Floribunda Lectin (WFA, WFL), Biotinylated

Wisteria Floribunda Lectin (WFA, WFL), Unconjugated Catalog #: L-1350-5

Wisteria Floribunda Lectin (WFA, WFL), Biotinylated

N-acetylgalactosamine Catalog #: S-9001

Citations

Wisteria Floribunda Lectin (WFA, WFL), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

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