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Maackia Amurensis Lectin I (MAL I), Fluorescein
Description
Maackia amurensis?lectin I binds gal (β-1,4) glcNAc but tolerates substitution of?N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.
Fluorescein labeled?Maackia amurensis?lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 μg/ml. |
| Recommended Storage | 2-8°C |
| Maximum Excitation | 495-500 nm |
| Inhibiting and/or Eluting Sugar | 200 mM lactose (S-9004) |
| Maximum Emission | 514-521 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2 |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Sugar Specificity | Galactose, Lactose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Maackia Amurensis Lectin II (MAL II), Unconjugated Catalog #: L-1260-2
Maackia Amurensis Lectin I (MAL I), Unconjugated Catalog #: L-1310-5
Lactose Catalog #: S-9004
Maackia Amurensis Lectin I (MAL I), Biotinylated Catalog #: B-1315-2
Maackia Amurensis Lectin II (MAL II), Biotinylated Catalog #: B-1265-1
Citations
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Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011
Inhibiting Sugar: 200 mM lactose