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Vector Labs 公司是世界著名的生物學(xué)檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標(biāo)記、分離與檢測試劑。
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI
Still using VectaShield? HardSet™ with DAPI?
Try our upgraded product VECTASHIELD? Vibrance™ Antifade Mounting Medium with DAPI
Description
VECTASHIELD HardSet?Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.?VECTASHIELD?HardSet Mounting Medium with DAPI hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.?
Features:
- Inhibits photobleaching of?fluorescent dyes and fluorescent proteins
- Ideal refractive index (1.46)
- Ready-to-use – from the refrigerator to the benchtop
- No warming necessary
- Can be stored without sealing for long term analysis
- Hardening formulation
- DAPI counterstain
Specifications
| Unit Size | 10 ml |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Cellular Imaging |
| Mounting | Aqueous (Hardening) |
| Antifade | Yes |
| Counterstain | DAPI (blue fluorescence) |
| Cellular Stain | Nucleus |
Documents
- VECTASHIELD Fluorochrome Compatibility
- User Guide
- In Situ Hybridization Detection Protocol
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I am using VECTASHIELD? HardSet™ Mounting Medium with DAPI (Cat No. H-1500). The sections look good initially, however I do see bubble or vacuole formation occur under the coverslip after some time. Do you have any suggestions on how this situation can be remedied?
Fixed, thin cut (<10 um), glass mounted sections are recommended to be used in combination with this medium. Unfixed material, thicker sections or the use of gasket or chamber slides or well formats may increase the incidence of bubble formation when applying H-1500. If using thin sections as described, ensure an adequate volume of the medium is being applied to spread out under the coverslip. Storage of slides in the freezer (-20 °C) may also reduce the incidence of bubbles. For existing mounted sections with bubble formation, coverslips can be removed by soaking the slides in buffer, and then remounted using fresh media.
Does VECTASHIELD contain glycerol?
All of the VECTASHIELD formats we currently offer which includes regular non-setting VECTASHIELD, PLUS, HardSet and Vibrance formulations each contain a percentage of glycerol.
Do I need to dehydrate the tissue sections before applying VECTASHIELD?
No form of tissue dehydration (e.g. air drying or ethanol exposure) is required nor recommended when applying VECTASHIELD. From our experience, the most optimal antifade actions of VECTASHIELD are obtained when the preparation is removed from the final buffer/water rinse, kept slightly wet/moist and then coverslipped with a small volume (25-50 uL) of VECTASHIELD.
Do I need to seal the perimeter of the coverslip after I apply VECTASHIELD?
That depends upon how long you wish to retain the slides and which VECTASHIELD formulation you are using. If you are using one of our non-setting VECTASHIELD products such as H-1000/H-1200 or H-1900/H-2000, then we suggest sealing the coverslip with plastic sealant or nail polish if you intend to keep the slides beyond a day or so. If you are using one of our setting/curing formulations such as VECTASHIELD HardSet or Vibrance, then in most cases when using thin cut (<10 um) tissue sections or cell monolayers, no sealing of the coverslip is requuired.
Related Products
VECTASHIELD? Vibrance™ Antifade Mounting Medium Catalog #: H-1700
VECTASHIELD? Vibrance™ Antifade Mounting Medium with DAPI Catalog #: H-1800
VECTASHIELD? PLUS Antifade Mounting Medium Catalog #: H-1900
VECTASHIELD? PLUS Antifade Mounting Medium with DAPI Catalog #: H-2000
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Citations
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VECTASHIELD?Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (in the "Documents" section) to determine if VECTASHIELD?will be compatible in your system.
All formulations of?VECTASHIELD Antifade?Mounting Media are available with or without the counterstain DAPI (4', 6-diamidino-2-phenylindole). ?The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD?Mounting Medium?without DAPI. ?DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.
R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.
The refractive index for VECTASHIELD?Mounting Medium is 1.45.
VECTASHIELD?Mounting Medium in Super Resolution Microscopy
The optimal medium for super-resolution imaging methods maximizes the lifetime and photoswitching characteristics of fluorophores. VECTASHIELD?Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIM imaging, such as Cy5 or Alexa Fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899; Glushonkov, O. et al., 2018, Scientific Reports, Vol.?8,?8749?(2018)?).
VECTASHIELD?Mounting Medium Antifade Comparison
Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD?mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. ?Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.
