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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命，該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。
VECTASHIELD? PLUS Antifade Mounting Medium with DAPI
Description
VECTASHIELD PLUS?Antifade Mounting Medium?with DAPI is a unique, stable formula for preserving fluorescence. This medium inhibits?rapid photobleaching of fluorescent proteins and fluorescent dyes.?
Features:
- No inherent toning or background
- Superior signal retention across the spectrum from blue to far red
- Mounted sections can be viewed in 1 hour
- DAPI (blue) counterstain premixed with medium
- Available in two convenient sizes
- Superior non-setting formulation
VECTASHIELD PLUS?Mounting Medium?with DAPI does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 2-8 °C, protected from light.
Specifications
| Unit Size | 2 ml, 10 ml |
|---|---|
| Applications | Immunofluorescence, In situ hybridization |
| Mounting | Aqueous (Non-Hardening) |
| Antifade | Yes |
| Counterstain | DAPI (blue fluorescence) |
Documents
- User Guide
- Safety Data Sheet
- VECTASHIELD Fluorochrome Compatibility
- Download CoA
- Datasheet
Related Products
VECTASHIELD? PLUS Antifade Mounting Medium Catalog #: H-1900
VECTASHIELD? Vibrance™ Antifade Mounting Medium with DAPI Catalog #: H-1800
VECTASHIELD? Vibrance™ Antifade Mounting Medium Catalog #: H-1700
VECTASHIELD? Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD? Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
Technical Information
The refractive index for VECTASHIELD?Mounting Medium is 1.45.
VECTASHIELD PLUS?Mounting Medium does not solidify, but rather, remains a liquid on the slide ‐ which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.
VECTASHIELD?Mounting Medium Antifade Comparison
Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD?mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer's published results.
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VECTASHIELD PLUS?References:
1.?González-Rodríguez, P. et al.?(2020)?The DNA methyltransferase DNMT3A contributes to autophagy long-term memory. Autophagy. (https://www.tandfonline.com/doi/full/10.1080/15548627.2020.1816664)
2.?Viena, T. et al. (2020) Calretinin and calbindin architecture of the midline thalamus associated with prefrontal–hippocampal circuitry. Hippocampus (https://onlinelibrary.wiley.com/doi/abs/10.1002/hipo.23271)
3.?Molina-García, L. et al. (2020) Direct glia-to-neuron transdifferentiation gives rise to a pair of male-specific neurons that ensure nimble male mating. eLife (https://elifesciences.org/articles/48361)
4. Valle, L. et al. (2021) Induction of Brain Tumors by the Archetype Strain of Human Neurotropic JCPyV in a Transgenic Mouse Model. Viruses (https://www.mdpi.com/1999-4915/13/2/162/htm)
