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Soybean Agglutinin (SBA), Unconjugated
Description
SBA preferentially binds to oligosaccharide structures with terminal α- or β-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance.
Specifications
| Unit Size | 10 mg, 25 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation |
| Recommended Usage | Although many buffers can be employed for reconstituting and diluting this lectin, we recommend 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2. For preserving solutions stored at 4 oC, 0.08% sodium azide can be used. |
| Recommended Storage | 2-8 °C |
| Conjugate | Unconjugated |
| Sugar Specificity | Galactose, N-Acetylgalactosamine |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Soybean Agglutinin (SBA), Biotinylated Catalog #: B-1015-5
Soybean Agglutinin (SBA), Fluorescein Catalog #: FL-1011-2
N-acetylgalactosamine Catalog #: S-9001
Soybean Agglutinin (SBA), Agarose bound Catalog #: AL-1013-2
Citations
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An important application for SBA is the separation of pluripotent stem cells from human bone marrow. Cells fractionated by SBA do not produce graft vs host disease and can be used in bone marrow transplantation across histocompatibility barriers.
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine