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Lens Culinaris Agglutinin (LCA), Unconjugated
Description
LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.
Specifications
| Unit Size | 10 mg, 25 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation |
| Recommended Usage | Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 oC, 0.08% sodium azide can be used. |
| Recommended Storage | 2-8 °C |
| Inhibiting and/or Eluting Sugar | mixture of 200 mM α?methylmannoside/200 mM α?methylglucoside |
| Conjugate | Unconjugated |
| Sugar Specificity | Mannose, Glucose |
Documents
- Safety Data Sheet
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Download CoA
- Datasheet
Related Products
Lens Culinaris Agglutinin (LCA), Biotinylated Catalog #: B-1045-5
Lens Culinaris Agglutinin (LCA), Fluorescein Catalog #: FL-1041-5
α-methylmannoside Catalog #: S-9005
α-methylglucoside Catalog #: S-9006
Citations
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Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside