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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命，該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。
Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U.
Description
The Ready-to-Use (R.T.U.) Biotinylated Horse Anti-Goat IgG Antibody?is?used in an avidin-biotin or streptavidin-biotin detection system.?These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
- Suitable for use with goat, sheep, and bovine IgG primary antibodies
- Recognizes both heavy and light chains (H+L)?
Specifications
| Unit Size | 50 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | Ready-to-use solution of biotinylated horse anti-goat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative. |
| Recommended Storage | 2-8 °C |
| Recommended Usage | Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Goat IgG and incubate for 30 minutes at room temperature.Wash inbuffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN? Elite? ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl. |
| Target Species | Goat |
| Conjugate | Biotinylated |
| Host Species | Horse |
| Format | Ready-to-Use |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Technical Information
Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.
The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker? solution (Cat. No. SP-5030) is recommended.