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Vector Labs 公司是世界著名的生物學(xué)檢測試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標(biāo)記、分離與檢測試劑。
VectaFluor™ Duet Immunofluorescence Double Labeling Kit, DyLight? 488 Anti-Rabbit (green), DyLight? 594 Anti-Mouse (red)
Description
VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight?fluorescent dyes in a single step. ?Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight?dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.
Features:
- Robust cocktail formulation of DyLight anti-mouse IgG/anti-rabbit IgG secondary antibodies
- Affinity-purified, ultrapure, high affinity antibodies
- Secondary detection reagent optimally labeled with DyLight dyes to provide the brightest label for fluorescence microscopy
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunofluorescence |
| Target Species | Universal (Mouse/Rabbit) |
| Conjugate | DyLight 488/DyLight 594 |
| Color of Fluorescence | Kit (Green, Red) |
| Host Species | Horse |
| Format | Ready-to-Use |
Kit Components
Kit Contents:
- 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
- 15 ml VectaFluor Duet Reagent [DyLight 488 Anti-Rabbit IgG and DyLight 594 Anti-Mouse IgG cocktail (rabbit-green, mouse-red)] made in horse, ready-to-use in 5% NHS.
DyLight? 488:
- Excitation maximum: 493 nm
- Emission maximum: 518 nm
- Color: Green
DyLight?594:
- Excitation maximum: 592 nm
- Emission maximum: 617 nm
- Color: Red
Documents
- Safety Data Sheet
- User Guide
- Download CoA
- Datasheet
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VECTASHIELD? Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD? HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD? HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Technical Information
Kit Contents:
- 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking?
- 15 ml VectaFluor Duet Reagent [DyLight?488 Anti-Rabbit IgG and DyLight?594 Anti-Mouse IgG cocktail (rabbit-green, mouse-red)] made in horse, ready-to-use in 5% NHS.?
DyLight?488:
- Excitation maximum: ? 493 nm
- Emission maximum: ?518 nm
- Color: ?Green
DyLight?594:
- Excitation maximum: ? 592 nm
- Emission maximum: ?617 nm
- Color: ?Red
DyLight?fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. ?
DyLight?dyes offer a number of potential advantages including greater photostability and brighter fluorescence. ?DyLight?dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight?dyes can be applied as single labels or in combination with other DyLight?dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. ?The Dylight?dyes currently offered are DyLight?488 (green), DyLight?549 (orange), DyLight?594 (red), and DyLight?649 (far red).
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