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Vector Labs 公司是世界著名的生物學檢測試劑公司，七十年代其在全球首推的生物素-親和素系統引領了生物學檢測方法的重大革命，該系統被普遍視為目前最靈敏、最可靠與最有效的染色系統，并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統仍在不斷地發展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商，并提供大量的新產品，如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。
VectaFluor™ Duet Immunofluorescence Double Labeling Kit, DyLight? 594 Anti-Rabbit (red), DyLight? 488 Anti-Mouse (green)
Description
VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight?fluorescent dyes in a single step. ?Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight?dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.
Features:
- Robust cocktail formulation of DyLight anti-mouse IgG/anti-rabbit IgG secondary antibodies
- Affinity-purified, ultrapure, high affinity antibodies
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Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunofluorescence |
| Target Species | Universal (Mouse/Rabbit) |
| Conjugate | DyLight 488/DyLight 594 |
| Color of Fluorescence | Kit (Green, Red) |
| Host Species | Horse |
| Format | Ready-to-Use |
Kit Components
Kit Contents:
- 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
- 15 ml VectaFluor Duet Reagent [DyLight 594 Anti-Rabbit IgG and DyLight 488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse
Documents
- Safety Data Sheet
- User Guide
- Download CoA
- Datasheet
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Technical Information
Kit Contents:
- 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking?
- 15 ml VectaFluor Duet Reagent [DyLight?594 Anti-Rabbit IgG and DyLight?488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse, ready-to-use in 5% NHS.?
DyLight? 488:
- Excitation maximum: ? 493 nm
- Emission maximum: ?518 nm
- Color: ?Green
DyLight? 594:
- Excitation maximum: ? 592 nm
- Emission maximum: ?617 nm
- Color: ?Red
DyLight?fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. ?
DyLight?dyes offer a number of potential advantages including greater photostability and brighter fluorescence. ?DyLight?dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight?dyes can be applied as single labels or in combination with other DyLight?dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. ?The Dylight?dyes currently offered are DyLight?488 (green), DyLight?549 (orange), DyLight?594 (red), and DyLight?649 (far red).
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