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Vector Labs 公司是世界著名的生物學(xué)檢測(cè)試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測(cè)方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類與核酸的標(biāo)記、分離與檢測(cè)試劑。
Animal-Free Blocker? and Diluent, R.T.U.
Description
R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding?in immunohistochemical applications. This product contains no animal-derived proteins.?The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.?
Specifications
| Unit Size | 100 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot |
| Recommended Storage | 2-8 °C |
| Recommended Usage | This product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications.This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 oC for up to a week. |
| Blocking Action | Non-Specific Protein Blocking |
| Format | Ready-to-Use |
Documents
- Safety Data Sheet
- User Guide
- Download CoA
- Datasheet
Product FAQs
What is the difference between the R.T.U. Animal Free Block and Diluent (SP-5035) and the 5x Animal Free Blocker? (SP-5030)?
Related Products
Carbo-Free Blocking Solution (10x Concentrate) Catalog #: SP-5040-125
Bovine Serum Albumin (BSA), Immunohistochemical Grade Catalog #: SP-5050-500
10x Casein Solution Catalog #: SP-5020-250
Animal-Free Blocker?, 5X Concentrate Catalog #: SP-5030-250
Technical Information
This plant protein derived product is a universal antibody diluent and blocking reagent intended for cell and tissue based immunohistochemistry (IHC) and immunofluorescent (IF) applications. This ready-to-use solution can be used as an alternative to normal sera, BSA, casein and non-fat dry milk in methods where these or other blocking agents have been found to be ineffective.
It is supplied without any sodium azide, and as such, can be used with both peroxidase and alkaline phosphatase antibody conjugates and all secondary detection reagents including polymer systems and avidin/biotin reagents that incorporate these enzymes.?
Importantly, this is a different formulation than our concentrated (5x) animal free blocker, due to the optimized conditions and neutral pH of the solution for IHC and IF methods.?