淀粉損失檢測(cè)試劑盒

淀粉損失檢測(cè)試劑盒,Starch Damage Assay Kit

英文名:Starch Damage Assay Kit

中文品名:淀粉損傷檢測(cè)試劑盒

貨號(hào):K-SDAM

規(guī)格:200 assays per kit,200 次檢測(cè)

應(yīng)用:淀粉損傷檢測(cè)試劑盒,用于谷物面粉中淀粉損傷的檢測(cè)和分析。

原理:
谷物淀粉中淀粉損傷紫外檢測(cè)方法
淀粉損傷檢測(cè)試劑盒 (K-SDAM)檢測(cè)原理
檢測(cè)方法:分光光度法 @510 nm
反應(yīng)時(shí)間:~ 40分鐘
檢測(cè)限:樣品重量的0.5-100%
應(yīng)用案例:
谷物面粉和其它食材。
方法識(shí)別:
符合AACC (方法 76-31.01), ICC (標(biāo)準(zhǔn)號(hào) 164) 和 RACI (標(biāo)準(zhǔn)方法)。
試劑盒組成:
Bottle 1: 真菌α-淀粉酶(pH5.4和40°C條件下,10 mL, 1,000 U/mL on Ceralpha reagent* )。硫酸銨懸浮液。
4°C下穩(wěn)定超過 3年
Bottle 2: 淀粉葡萄糖苷酶(pH 4.5 和40°C條件下,4 mL, 200 U/mL 在可溶性淀粉中)。硫酸銨懸浮液。
4°C穩(wěn)定超過3年
Bottle 3: GOPOD 試劑緩沖液。緩沖液(50 mL, pH7.4), 對(duì)羥基苯甲酸和疊氮鈉(0.095% w/v).
4°C穩(wěn)定超過4年
Bottle 4: GOPOD 試劑酶。葡萄糖氧化酶,過氧化酶和4-氨基安替比林。凍干粉。
-20°C穩(wěn)定超過5年
Bottle 5: D-葡萄糖標(biāo)準(zhǔn)溶液(5 mL, 1.5 mg/mL) 溶于0.2% (w/v)苯甲酸。
室溫下穩(wěn)定超過5年
Bottle 6: 小麥面粉標(biāo)樣。淀粉損傷程度在小瓶標(biāo)簽上注明。
室溫下穩(wěn)定超過5年

Megazyme 淀粉損傷檢測(cè)試劑盒(K-SDAM)

淀粉損傷檢測(cè)試劑盒, Starch Damage Assay Kit , 貨號(hào):K-SDAM
品牌:Megazyme
貨號(hào):K-SDAM
中文品名:淀粉損傷檢測(cè)試劑盒
品名:Starch Damage Assay Kit
規(guī)格:200 次檢測(cè)
應(yīng)用:淀粉損傷檢測(cè)試劑盒,用于谷物面粉中淀粉損傷的檢測(cè)和分析。
原理:
谷物淀粉中淀粉損傷紫外檢測(cè)方法
淀粉損傷檢測(cè)試劑盒 (K-SDAM)檢測(cè)原理
檢測(cè)方法:分光光度法 @510 nm
反應(yīng)時(shí)間:~ 40分鐘
檢測(cè)限:樣品重量的0.5-100%
應(yīng)用案例:
谷物面粉和其它食材。
方法識(shí)別:
符合AACC (方法 76-31.01), ICC (標(biāo)準(zhǔn)號(hào) 164) 和 RACI (標(biāo)準(zhǔn)方法)。
試劑盒組成:
Bottle 1: 真菌α-淀粉酶(pH5.4和40°C條件下,10 mL, 1,000 U/mL on Ceralpha reagent* )。硫酸銨懸浮液。
4°C下穩(wěn)定超過 3年
Bottle 2: 淀粉葡萄糖苷酶(pH 4.5 和40°C條件下,4 mL, 200 U/mL 在可溶性淀粉中)。硫酸銨懸浮液。
4°C穩(wěn)定超過3年
Bottle 3: GOPOD 試劑緩沖液。緩沖液(50 mL, pH7.4), 對(duì)羥基苯甲酸和疊氮鈉(0.095% w/v).
4°C穩(wěn)定超過4年
Bottle 4: GOPOD 試劑酶。葡萄糖氧化酶,過氧化酶和4-氨基安替比林。凍干粉。
-20°C穩(wěn)定超過5年
Bottle 5: D-葡萄糖標(biāo)準(zhǔn)溶液(5 mL, 1.5 mg/mL) 溶于0.2% (w/v)苯甲酸。
室溫下穩(wěn)定超過5年
Bottle 6: 小麥面粉標(biāo)樣。淀粉損傷程度在小瓶標(biāo)簽上注明。
室溫下穩(wěn)定超過5年

優(yōu)點(diǎn):

價(jià)格非常具有競(jìng)爭(zhēng)力
所有試劑制備后可以穩(wěn)定保存超過2年
僅提供酶法檢測(cè)試劑盒
特異性
操作簡(jiǎn)單
官網(wǎng)提供Mega-Calc™ 軟件工具用于一站式原始數(shù)據(jù)處理
包含標(biāo)準(zhǔn)品

The Starch Damage test kit is suitable for the measurement and analysis of starch damage in cereal flours.

Colourimetric method for the determination of Starch Damage
in cereal flours

Principle:
(fungal α-amylase)
(1) Damaged (or gelatinised) starch + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 200 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 40 min
Detection limit: 0.5-100% of sample weight
Application examples:
Cereal flours and other materials
Method recognition:
AACC (Method 76-31.01), ICC (Standard No. 164), and RACI (Standard
Method)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Very specific
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
參考文獻(xiàn):
An improved enzymic method for the measurement of starch damage in wheat flour. Gibson, T. S., Al Qalla, H. & McCleary, B. V. (1992). Journal of Cereal Science, 15(1), 15-27.
Collaborative evaluation of an enzymatic starch damage assay kit and comparison with other methods. Gibson, T. S., Kaldor, C. J. & McCleary, B. V. (1993). Cereal Chem., 70(1), 47-51.
Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.
Measurement of carbohydrates in grain, feed and food. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.
Starch properties, in vitro digestibility and sensory evaluation of fresh egg pasta produced from oat, teff and wheat flour. Hager, A. S., Czerny, M., Bez, J., Zannini, E. & Arendt, E. K. (2013). Journal of Cereal Science, 58(1), 156-163.
Effect of sorghum flour composition and particle size on quality properties of gluten-free bread. Trappey, E. F., Khouryieh, H., Aramouni, F. & Herald, T. (2014). Food Science and Technology International, 1082013214523632.
Nutritional properties and ultra-structure of commercial gluten free flours from different botanical sources compared to wheat flours. Hager, A. S., Wolter, A., Jacob, F., Zannini, E. & Arendt, E. K. (2012). Journal of Cereal Science, 56(2), 239-247.
Quality variations in flours used for pretzel manufacturing. Yao, N. & Seetharaman, K. (2010). International Journal of Food Science & Technology, 45(10), 2052-2061.
Effect of corn preparation methods on dry-grind ethanol production by granular starch hydrolysis and partitioning of spent beer solids. Lamsal, B. P., Wang, H. & Johnson, L. A. (2011). Bioresource Technology, 102(12), 6680-6686.
Flaking as a corn preparation technique for dry-grind ethanol production using raw starch hydrolysis. Lamsal, B. P. & Johnson, L. A. (2012). Journal of Cereal Science, 56(2), 253-259.
Chemical composition and functional properties of native chestnut starch (Castanea sativa Mill). Cruz, B. R., Abra?o, A. S., Lemos, A. M. & Nunes, F. M. (2013). Carbohydrate Polymers, 94(1), 594-602.
Changes in rice with variable temperature parboiling: thermal and spectroscopic assessment. Himmelsbach, D. S., Manful, J. T. & Coker, R. D. (2008). Cereal chemistry, 85(3), 384-390.
Determination of formulation and processing factors affecting slowly digestible starch, protein digestibility and antioxidant capacity of extruded sorghum–maize composite flour. Licata, R., Chu, J., Wang, S., Coorey, R., James, A., Zhao, Y. & Johnson, S. (2014). International Journal of Food Science & Technology, 49(5), 1408-1419.
Analysis of starch amylolysis using plots for first-order kinetics. Butterworth, P. J., Warren, F. J., Grassby, T., Patel, H. & Ellis, P. R. (2012). Carbohydrate Polymers, 87(3), 2189-2197.