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MagnaLINK? Streptavidin Magnetic Beads (2.8 μm)
Description
MagnaLINK 2.8 μm Streptavidin Magnetic Beads offer the highest biotin binding capacity on the market, combined with exceptional size uniformity. Higher binding translates to reduced bead mass required to immobilize a biotinylated sample and lower background from nonspecific binding, resulting in better signals and lower costs. MagnaLINK Streptavidin Magnetic Beads are 2.8 micron diameter, super-paramagnetic, hydrophilic polymer–encapsulated (no exposed iron), monodispersed microspheres with a uniform size distribution and a fast (<2 min) magnetic response time. They are colloidally stable with or without non-ionic detergents. The key to high biotin binding is in the unique covalently crosslinked streptavidin, based on ChromaLINK? technology. The high surface area, when combined with our efficient linking chemistry, produces a consistent product with a free biotin binding capacity of at least 12 nmol per milligram of beads. MagnaLINK beads also demonstrate exceptional size uniformity of <5% CV, as evidenced by scanning electron microscopy (SEM). These combined technologies make MagnaLINK beads the ideal magnetic bead for high-throughput robotic applications.
Specifications
| Bead Size | 2.8 μm |
|---|---|
| Loading Capacity | ≥ 10 nmol/mg |
| Reactivity | Biotin |
| Applications | Antibody Labeling, Aptamers, Next-Generation Sequencing (NGS), Photocrosslinking Studies |
| Recommended Storage | 2° – 8°C – Do Not Freeze |
Documents
- User Guide
- Safety Data Sheet
- Bioconjugation White Paper
- Download CoA
- Datasheet
Citations
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Product Description
MagnaLINK Streptavidin Magnetic beads are highly uniform, 2.8 µmeter polymer-encapsulated (no exposed iron), super-paramagnetic beads containing covalently cross-linked streptavidin (Figure 1). MagnaLINK streptavidin magnetic beads are made by covalently cross-linking streptavidin to a hydrophilic surface using the SoluLINK conjugation technology. The high surface area of these paramagnetic beads when combined with the efficient SoluLINK linking technology produces the most consistent and highest biotin binding capacity (≥10 nmol/mg) of any uniform streptavidin magnetic bead on the market. Beads are supplied at 1% solids (10 mg/mL) in nuclease-free water with 0.05% sodium azide.
Features of our MagnaLINK beads
- Highest free biotin binding capacity of any uniform bead (≥ 10 nmol/mg)
- Binds 0.8 nmol/mg biotinylated oligonucleotide
- Binds 0.75 nmol/mg biotinylated-IgG @ 4 biotins/IgG
- Beads are encapsulated (no exposed iron)
- Paramagnetic beads are highly uniform in size (2.8 ± 0.2 microns)
- Fast magnetic response time (60% w/w magnetite)
Binding Capacity Comparison: MagnaLINK Streptavidin Magnetic Bead Vs. Competitor’s Bead of Similar Size
| Ligand | MagnaLINK 2.8 μm Binding Capacity | Competitor’s 2.8 μm Bead Binding Capacity |
| Free biotin | ≥ 10 nmol/mg | 0.90 nmol/mg |
| Biotinylated Oligo (23-mer) | ≥ 0.8 nmol/mg | N/A |
| Biotinylated IgG (4 biotins per IgG) | 750 pmol/mg (113 μg/mg) | 60 pmol/mg (10 μg/mg) |
MagnaLINK References:
- Moore R, Worrallo M, Mitchell P, Harriman J, Glen K, Thomas R. Immobilisation of Delta-like 1 ligand for the scalable and controlled manufacture of hematopoietic progenitor cells in a stirred bioreactor. BMC Biotechnology volume 17, Article number: 65 (2017).
Intellectual Property
SoluLINK? Bioconjugation For Research Use Only. Not for use in diagnostic procedures. For additional licensing restrictions, please see the license agreement at vectorlabs.com/solulink-research-license.
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.