Vector Labs代理，歡迎訪問(wèn)Vector Labs官網(wǎng)或者咨詢(xún)我們獲取相關(guān)Vector Labs產(chǎn)品信息以及報(bào)價(jià)。
Vector Labs 公司是世界著名的生物學(xué)檢測(cè)試劑公司，七十年代其在全球首推的生物素-親和素系統(tǒng)引領(lǐng)了生物學(xué)檢測(cè)方法的重大革命，該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng)，并被廣泛應(yīng)用于免疫組織化學(xué)、免疫電鏡、原位雜交與凝集素化學(xué)中。該系統(tǒng)仍在不斷地發(fā)展，以滿(mǎn)足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產(chǎn)品的主要供應(yīng)商，并提供大量的新產(chǎn)品，如酶底物、神經(jīng)元示蹤劑及蛋白質(zhì)、糖類(lèi)與核酸的標(biāo)記、分離與檢測(cè)試劑。
ImmPRESS? HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase
Description
The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The?Inclusion of BLOXALL? endogenous enzyme quenching solution and ImmPACT? DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product.?
Features:
- High?sensitivity and low background
- Enhanced accessibility to nuclear and membrane antigens
- Ready-to-use in a convenient dropper bottle
- Shorter assay time
- Simplified multiple labeling
- Designed for use with both rabbit and mouse primary antibodies
?
The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.
Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.
Specifications
| Unit Size | 1 Kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs |
| Technology | Micropolymer Reagents |
| Detection Enzyme(s) | Peroxidase |
| Conjugate | Micropolymer HRP |
| Target Species | Universal (Mouse/Rabbit) |
| Format | Ready-to-Use |
Kit Components
Kit Contents:
- 15 ml ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
- 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
- 15 ml BLOXALL Endogenous Enzyme Blocking Solution
- ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)
Documents
- Safety Data Sheet
- User Guide
- ImmPRESS Polymer & Automated Staining App. Note
- ImmPRESS Troubleshooting Guide
- Download CoA
- Datasheet
Technical Information
The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.
Staining Procedure
The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.
When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:
- Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
- Use the M.O.M.? ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.
?
Learn more about the?ImmPRESS Polymer Detection Systems?in double labeling.
Product review on?Biocompare.com.