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Wheat Germ Agglutinin (WGA), Unconjugated
Description
The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).
Specifications
| Unit Size | 10 mg, 25 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation |
| Recommended Usage | Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 oC, 0.08% sodium azide can be used. |
| Recommended Storage | 2-8 °C |
| Conjugate | Unconjugated |
| Sugar Specificity | N-Acetylglucosamine |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Wheat Germ Agglutinin (WGA), Peroxidase Catalog #: PL-1026-2
N-acetylglucosamine Catalog #: S-9002
Chitin Hydrolysate Catalog #: SP-0090-10
Wheat Germ Agglutinin (WGA), Biotinylated Catalog #: B-1025-5
Citations
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Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required