AMCA (7-amino-4-methylcoumarin-3-acetic acid) labeled Goat Anti-Human Kappa Chain Antibody is a?chain-specific antibody, which?distinguishes between chains or classes of target immunoglobulins. This kappa chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.

Features:

• Optimally labeled with AMCA to provide the brightest label for fluorescence microscopy
• Can be used for flow cytometry or used for tissue staining?
• Supplied in solution?
• Excitation: 350 nm?
• Emission: 450 nm
• Color: Blue

VectaFluor R.T.U. DyLight 594 Anti-Rabbit IgG?can be used?for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

• Ready-to-use format?offers maximum convenience for fluorescence staining of cells and tissues
• Recognizes both heavy and light chains (H+L)?
• Optimally labeled with DyLight?594 to provide the brightest label for fluorescence microscopy?
• Excitation: 592 nm?
• Emission: 617 nm?
• Color: Red

Kit Contents:

• 15 ml pre-diluted, stabilized secondary antibody solution?
• 15 ml 2.5% normal horse serum for blocking

VectaFluor Ready-to-Use (R.T.U.) DyLight dye-labeled Secondary Antibodies offer maximum convenience for fluorescence staining of cells and tissues. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight?dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity.

VectaFluor R.T.U. Antibody Reagents are supplied as 15 ml pre-diluted, stabilized, ready-to-use solutions along with 15 ml ready-to-use 2.5% normal horse serum for blocking.

DyLight?fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. ?

DyLight?dyes offer a number of potential advantages including greater photostability and brighter fluorescence. ?DyLight?dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight?dyes can be applied as single labels or in combination with other DyLight?dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. ?The Dylight?dyes currently offered are DyLight?488 (green), DyLight?549 (orange), DyLight?594 (red), and DyLight?649 (far red).

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Features:

• Affinity-purified, ultrapure, high affinity antibody?
• Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species?
• Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)?
• Optimally labeled with DyLight^? 594 to provide the brightest label for fluorescence microscopy?
• Supplied in solution?
• Excitation: 592 nm?
• Emission: 617 nm?
• Color: Red

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).

DyLight^??fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. ?

DyLight^??dyes offer a number of potential advantages including greater photostability and brighter fluorescence. ?DyLight^??dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight^??dyes can be applied as single labels or in combination with other DyLight^??dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. ?The Dylight^??dyes currently offered are DyLight^??488 (green), DyLight^??549 (orange), DyLight^??594 (red), and DyLight^??649 (far red).

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